Caractérisation des réponses humorales mucosales dirigées contre les virus à ARN double brin

 

Valerie Abadie

Centre de recherche du CHU Ste-Justine

 

Domaine : maladies infectieuses et immunitaires

Programme chercheurs nationaux

Concours 2014-2015

Certain classes of viruses, in particular dsRNA viruses, are not only the most common cause of life-threatening gastroenteritis in children and infants worldwide, but are also thought to trigger autoimmune diseases. Although the diarrhea caused by these viruses is considered to be a vaccine-preventable disease, it has been difficult in practice to develop therapies that work as effectively in both industrialized and developing countries primarily because immunity to such viruses has been poorly characterized. We are convinced that a better understanding of the immune response in the gut following oral infection or vaccination and understanding the cross talk between mucosal and systemic immunity should expedite the development of such vaccines.

This proposal aims at understanding the nature of the antibody response induced at intestinal surfaces in response to an oral infection using reovirus as an infectious model system. In particular, we will focus on the impact of reovirus on intestinal B cell populations and on the induction of humoral antibody responses and compare this response to the one observed during systemic dsRNA virus administration.

This project will next investigate how humoral immune responses develop in the intestinal environment following oral reovirus infection. This work will use mice models and genetically modified viral strains that will help understand the mechanisms underlying local immune responses in the gut. Elucidation of how the immune system reacts to an orally ingested microbe constitutes a key step towards the development of novel vaccine formulations against these infectious agents.